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. 2005 Sep;54(9):1217–1223. doi: 10.1136/gut.2004.059998

Figure 4.

Figure 4

 Interferon γ (IFN-γ) ELISPOT responses of peripheral blood mononuclear cells (PBMC) from individual HLA-DQ2+ healthy subjects after a gluten free diet (GFD) for four weeks (n = 9) (A) and coeliac disease (CD) subjects on a long term GFD (n = 12) (D) to tissue transglutaminase (tTG) deamidated gliadin (500 μg/ml) before and on day 6 and day 10 after commencing a three day gluten challenge. IFN-γ ELISPOT responses of the same healthy (B) and CD (E) subjects to gliadin (500 μg/ml) with or without tTG deamidation (mean (SEM)). Individual IFN-γ ELISPOT responses to the p57–73 QE65 homologue, AG01 E9 (20 μg/ml) (see table 1 ▶ for sequence), in healthy (C) and CD (F) subjects. Individual IFN-γ ELISPOT responses of all HLA-DQ2+ CD subjects on a long term GFD (n = 59) to medium alone or p57–73 QE65 (25 or 50 μg/ml), AG01 E9 (20 μg/ml), or AG02 E9 (100 μg/ml) (see table 3 ▶ for sequences) (G), and according to HLA-DQ genotype (H). IFN-γ ELISPOT responses to tTG deamidated gliadin (500 μg/ml) on day 6 after a three day gluten challenge correlated with p57–73 QE65 in 17 individual CD subjects (I). SFU, spot forming units.