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. 2005 Sep;54(9):1309–1317. doi: 10.1136/gut.2005.065086

Table 1.

 Primer sequences used for generation of luciferase expression constructs

Sense primer 5′ – CAGATTGGGACTTCAACCCCGTC – 3′
1–as 5′ - CGCTGCCTCGAGGTTCGTCACAGGGTCCCCAGT – 3′
2–as 5′ - TACTCGAGGAGGCAATATTCGGAGC – 3′
3–as 5′ - TACTCGAGGACTCTGGGATCCTGC – 3′
4–as 5′ - TACTCGAGGGTGGAAGGCAGTGG– 3′
5–as 5′ - CCACTGCATGGCTCGAGGATGACTG – 3′
1B–as 5′ - TACTCGAGCAGTCCTCGCGGAGATTGACGA – 3′
1Bmut–as 5′ - TACTCGAGCAGTCCTCGCGGAGATTCCCGA – 3′
1 mut/wt–as 5′ - TACTCGAGGTTCGTCACAGGGTCCCCAGTCCTCGCGGAGATTCCCGAG – 3′
1 wt/mut–as 5′ - TACTCGAGGTTCGGGGCAGGGTCCCCAGTCCTCGCGGAGATTGACGAG – 3′
1 mut/mut–as 5′ - TACTCGAGGTTCGGGGCAGGGTCCCCAGTCCTCGCGGAGATTCCCGAG – 3′
3-mut-I–as 5′ - TACTCGAGACTCTAACATCCTGCAGAGT – 3′
3-mut-II–as 5′ - TACTCGAGACTCTGGGATATTCCTGCAGAGT – 3′

Mutated (mut) sequences are underlined.

as, antisense; wt, wild-type.