Table 1.
Sense primer | 5′ – CAGATTGGGACTTCAACCCCGTC – 3′ |
1–as | 5′ - CGCTGCCTCGAGGTTCGTCACAGGGTCCCCAGT – 3′ |
2–as | 5′ - TACTCGAGGAGGCAATATTCGGAGC – 3′ |
3–as | 5′ - TACTCGAGGACTCTGGGATCCTGC – 3′ |
4–as | 5′ - TACTCGAGGGTGGAAGGCAGTGG– 3′ |
5–as | 5′ - CCACTGCATGGCTCGAGGATGACTG – 3′ |
1B–as | 5′ - TACTCGAGCAGTCCTCGCGGAGATTGACGA – 3′ |
1Bmut–as | 5′ - TACTCGAGCAGTCCTCGCGGAGATTCCCGA – 3′ |
1 mut/wt–as | 5′ - TACTCGAGGTTCGTCACAGGGTCCCCAGTCCTCGCGGAGATTCCCGAG – 3′ |
1 wt/mut–as | 5′ - TACTCGAGGTTCGGGGCAGGGTCCCCAGTCCTCGCGGAGATTGACGAG – 3′ |
1 mut/mut–as | 5′ - TACTCGAGGTTCGGGGCAGGGTCCCCAGTCCTCGCGGAGATTCCCGAG – 3′ |
3-mut-I–as | 5′ - TACTCGAGACTCTAACATCCTGCAGAGT – 3′ |
3-mut-II–as | 5′ - TACTCGAGACTCTGGGATATTCCTGCAGAGT – 3′ |
Mutated (mut) sequences are underlined.
as, antisense; wt, wild-type.