Figure 7.

 Effects of pancreatitis associated protein (PAP) on nuclear factor κB (NFκB) activation in THP-1 cells (A), HT29 cells (B), and human umbilical vein endothelial cells (C). Cells were preincubated for one hour with increasing PAP concentrations (0, 50, 100, 500, or 1000 ng/ml) and stimulated with 10 ng/ml tumour necrosis factor α (TNF-α) for 30 minutes. Nuclear extracts were obtained, and activated NFκB was measured using an oligonucleotide based specific ELISA (TransAM). NFκB activation in TNF-α stimulated cells was significantly reduced in a dose dependent manner by incubation with PAP. Results are expressed as NFκB-fold induction Experiments were performed in quadruplicate and all results are expressed as mean (SEM). *p<0.05 versus previous PAP dose; †p<0.05 versus TNF-α (−) PAP 0 ng/ml.