Table 1.
siRNA | Sequence | Comment |
Bcl1 | 5′-CAGGACCUCGCCGCUGCAGACC-3′ | Starting position: 1659 |
3′-CGGUCCUGGAGCGGCGACGUCUGG-5′ | ||
Bcl3 | 5′-GCCUUUGUGGAACUGUACGGCC-3′ | Starting position: 2047 |
3′-UACGGAAACACCUUGACAUGCCGG-5′ | ||
Bcl5 | 5′-CAGGACCUCGCCGCUGCAGAC-3′ | Starting position: 1659 |
3′-CGGUCCUGGAGCGGCGACGUCUG-5′ | ||
Bcl8 | 5′-GCCUUUGUGGAACUGUACGGC-3′ | Starting position: 2047 |
3′-UACGGAAACACCUUGACAUGCCG-5′ | ||
Bcl11 | 5′-UGUGGAUGACUGAGUACCUGA-3′ | Starting position: 1982 |
3′-GGACACCUACUGACUCAUGGACU-5′ | ||
Control1 | 5′-GAUGAGGAUCGUUUCGCAUGA-3′ | Eukaryotic kanamycin resistance protein |
3′-UCCUACUCCUAGCAAAGCGUACU-5′ | ||
Control2 | 5′-CCACAUGAAGCAGCACGACUU-3′ | EGFP expression vector |
3′-CUGGUGUACUUCGUCGUGCUGAA-5′ | ||
Control3 | 5′-ACGGCUAGCUGUGAAAGGUCC-3′ | 3′ UTR of hepatitis C virus |
3′-UCUGCCGAUCGACACUUUCCAGG-5′ |
Starting position indicates position of the first nt of the siRNA sense strand in the bcl-2 mRNA (accession No M13994). Specificity of the sequences was verified by a BLAST search against human and murine databases to minimise unspecific or off target effects.