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. 2005 Sep;54(9):1298–1308. doi: 10.1136/gut.2004.056192

Table 1.

 Sequences of short interfering RNAs (siRNAs)

siRNA Sequence Comment
Bcl1 5′-CAGGACCUCGCCGCUGCAGACC-3′ Starting position: 1659
3′-CGGUCCUGGAGCGGCGACGUCUGG-5′
Bcl3 5′-GCCUUUGUGGAACUGUACGGCC-3′ Starting position: 2047
3′-UACGGAAACACCUUGACAUGCCGG-5′
Bcl5 5′-CAGGACCUCGCCGCUGCAGAC-3′ Starting position: 1659
3′-CGGUCCUGGAGCGGCGACGUCUG-5′
Bcl8 5′-GCCUUUGUGGAACUGUACGGC-3′ Starting position: 2047
3′-UACGGAAACACCUUGACAUGCCG-5′
Bcl11 5′-UGUGGAUGACUGAGUACCUGA-3′ Starting position: 1982
3′-GGACACCUACUGACUCAUGGACU-5′
Control1 5′-GAUGAGGAUCGUUUCGCAUGA-3′ Eukaryotic kanamycin resistance protein
3′-UCCUACUCCUAGCAAAGCGUACU-5′
Control2 5′-CCACAUGAAGCAGCACGACUU-3′ EGFP expression vector
3′-CUGGUGUACUUCGUCGUGCUGAA-5′
Control3 5′-ACGGCUAGCUGUGAAAGGUCC-3′ 3′ UTR of hepatitis C virus
3′-UCUGCCGAUCGACACUUUCCAGG-5′

Starting position indicates position of the first nt of the siRNA sense strand in the bcl-2 mRNA (accession No M13994). Specificity of the sequences was verified by a BLAST search against human and murine databases to minimise unspecific or off target effects.