Table 3.

Progeny testing of two trichimeras from experimental sets I and III

Trichimera (Set)	Microsatellite bands of component colonies	Microsatellite bands of test colony	Crosses		n	Microsatellite bands of progeny	Expected results from cross if testicular winner was gamete donor, n	Observed results from cross, n	G test
			Egg donor	Sperm donor
A = B = C	*A = 1,3	0,1	TC	TRI	30	1,3	7.5	8	P > 0.1
(I)	B = 1					1,2	0	1
	C = 1,2					0,3	7.5	6
						0,2	0	0
						0,1 + 1,1	15.0	15
		0,1	TRI	TC	9	1,3	2.25	0	P > 0.1
						1,2	0	0
						0,3	2.25	4
						0,2	0	0
						0,1 + 1,1	4.50	5
B = F = G	B = 1	2,2	TC	TRI	29	2,3	14.5	14	P > 0.5
(III)	*F = 1,3					2,1	14.5	15
	G = 1
		0,1	TRI	TC	13	1,3	3.25	4	P > 0.5
						0,3	3.25	3
						0,1 + 1,1	6.50	6

Reciprocal crosses were performed in which the trichimera acted as either functional male or functional female. Microsatellite bands of component colonies and progeny are the bands observed on an autoradiograph when microsatellite locus PB41 is amplified. The banding patterns of test colonies were inferred from microsatellite analysis of tissues from the test colonies using locus PB41 and the results from the crosses. For example, microsatellite analysis of the third test colony suggested that its genotype could have been either 0,2 or 2,2. But given the genotypes of the progeny from the cross in which it participated, we inferred that its genotype was most likely 2,2. The last column gives results from a G test of the hypothesis that the testicular winner from each of the trichimeras was the gametic donor. ∗, testicular winner identified by the microsatellite analysis of sperm from the trichimera. TC, test colony; TRI, trichimera.