Figure 6.

 Cytokine secretion and proliferation of anti-CD3 stimulated CD4⁺CD62L⁺ cells isolated from cytosin-guanosin containing oligodeoxynucleotide (CpG-ODN) treated donor mice. Mice were treated with either CpG-ODN or GpG-ODN (each 10 µg/day over five days) or left untreated, and CD4⁺CD62L⁺ cells were isolated and incubated in anti-CD3 coated wells, as described in materials and methods, over 24 hours for cytokine detection (A) or 72 hours for ³H-thymidine incorporation (B). PBS, phosphate buffered saline; IL, interleukin; IFN-γ, interferon γ; TGF-β, transforming growth factor β. *Significantly different from both GpG-ODN or untreated mice. Incubations were performed in quadruplicate and results are representative of three independent experiments.