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. 2005 Nov;54(11):1565–1572. doi: 10.1136/gut.2004.061168

Figure 1.

Figure 1

 Expression and function of rtTA transgenes in FABP-rtTA and DRA-rtTA transgenic mice. (A) Schematic diagram of the TRE2IL-8 construct. (B) RNA was isolated from tissues of FABP-rtTA and DRA-rtTA mice or a CMV-rtTA transfected cell line. The level of rtTA mRNA was measured by a RNase protection assay in which presence of the rtTA message resulted in protection of a 310 bp band. The highest levels of rtTA mRNA were detected in the ileum and caecum from FABP-rtTA mice. (C) Tissue distribution of induced luciferase activity by DOX in FABP-rtTA × tetOluc mice. Tissues from FABP-rtTA × tetOluc double transgenic mice were harvested three days after DOX induction and homogenized. Luciferase activity was determined and normalised to protein concentration. The pattern of luciferase expression in a single animal is shown. A similar pattern was observed in four other double transgenic mice analysed by the same method.