Figure 2.

 Kinetics of IL-8 induction by DOX in the cecum of FABP-rtTA × TRE2IL-8 mice. FABP-rtTA × TRE2IL-8 double transgenic mice (two mice per time point) were induced with DOX in their drinking water and the caecum harvested from 1–5 days later. The IL-8 concentrations of tissue homogenates were determined by ELISA and normalised to protein concentration. The induction ratio (ratio of normalised IL-8 expression in induced double transgenic mice compared with the uninduced mice) is indicated in parentheses above the bar for each time point. A similar time course of DOX induced induction of IL-8 in the caecum was observed in a second experiment.