Figure 2.

 Western blotting representing serum antibodies to carbonic anhydrase (CA) IV in patients with AIP (case 1) and ICP (case 2). Protein staining profiles of the blots are shown on the left panel. Both patients showed significant signals for serum antibodies to CA IV-glutathione S-transferase (GST) fusion protein (arrows) but no detectable signal was observed for serum antibodies to GST alone (arrowhead). In case 1, several bands were concomitantly seen on the blots with both CA IV-GST and GST, which were probably derived by reactions to bacterial endogenous proteins acquired in this patient.