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Predominant supranuclear location of hephaestin (Hp) in duodenal enterocytes. Immunofluorescent staining of duodenal sections from C57BL/6J mice on control diets using an antiserum to the C terminus of Hp demonstrated predominantly supranuclear staining. (A) Hp alone (green). (B) Preincubation with Hp peptide. (C) Propidium iodide staining of nucleus (in red) and Hp staining (in green). (D) Immunolocalisation of Hp (green) and Na+,K+-ATPase (red).
