Table 2.

cDNA array overexpression ratio and quantitative real time RT-PCR* overexpression fold in malignant gastrointestinal stromal tumours (GISTs) compared with benign GISTs

Gene	VIL2	COL8	CCNB1	HMG2	TSG101	CENP-F	FAK	DYRK2
GeneBank accession No	X51521	X57527	M25753	X62534	X13293	U19769	L13616	Y13493
Chromosomal locus	6q25.3	3q12−q13.1	5q12	4q31	11p15	1q32–q41	8q24−qter	12q13.13
Sample
GIST4	9.1 (2.3)) [8]	3.4 (0.4)) [1.2]	3.1 (0.4) [1.9]	3.9 (1.1) [13.8]	3.6 (1.2) [1.1]	5.1 (1.7) [5.5]	5.8 (0.7) [2.9]	5.7 (0.5) [1.9]
GIST5	8.6 (3.1) [4.6]	9.7 (0.6) [11.4]	2.7 (0.9) [1]	2.9 (0.9) [18.3]	4.1 (0.5) [2.4]	1 (0.3) [1.4]	2.7 (0.2) [1]	2.7 (0.4) [1.2]
GIST6	6.8 (1.4) [3.7]	4.7 (0.8) [16]	6.2 (1.5) [24.2]	4.3 (1.7) [18.6]	3.2 (1.4) [2.4]	8.7 (2.7) [1.4]	4.9 (1.4) [2.6]	4.7 (0.8) [6.3]
GIST7	3.2 (1.1) [13.7]	5.4 (1.1) [59.6]	3.1 (1.5) [8.6]	3.2 (0.4) [7]	2.8 (0.6) [2.6]	2.5 (1.9) [8.2]	2.5 (0.6) [5.3]	2.5 (0.5) [5.7]
GIST8	9.8 (1.3) [13.7]	4.3 (0.9) [9.2]	6.1 (1.8) [12.1]	3.1 (0.4) [1.9]	2 (0.4) [1.5]	3.8 (1.1) [11.1]	5.2 (1.2) [1.9]	3.6 (0.7) [1.2]
GIST9	2.6 (0.7) [1.5]	0.8 (0.2) [0]	3.1 (0.8) [13.0]	4.7 (1.3) [6.4]	3.8 (0.6) [1.8]	4.8 (1.2) [10.9]	3.5 (0.7) [2.3]	1.3 (0.8) [1.7]

Gene expression alterations in six malignant GISTs (GIST 4–9) compared with expression of three benign GISTs (GIST 1–3) using cDNA arrays and quantitative real time reverse transcription-polymerase chain reaction (RT-PCR).

*Standard deviation for microarray overexpression fold is shown in parentheses and quantitative real time RT-PCR overexpression is shown in square parentheses.

VIL2, villin 2; COL8A1, collagen 8 alpha 1 subunit; CCNB1, G2/mitotic specific cyclin B1; HMG2, high mobility group protein; TSG101, TSG101 tumour susceptibility protein; CENP-F, CENP-F kinetochore protein; FAK, protein tyrosine kinase 2; DYRK2, protein kinase DYRK2.