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. 2003 Feb;52(2):275–282. doi: 10.1136/gut.52.2.275

Figure 4.

Figure 4

Tissue inhibitor of metalloproteinase (TIMP) secretion by pancreatic stellate cells (PSCs). (A) TIMP1 and TIMP2 activity was analysed using reverse zymography. Lane 1: Human recombinant TIMP1. Lane 2: Human recombinant TIMP2. Lanes 3–5: PSC secretions obtained from three separate cell preparations after 24 hours of culture with serum free culture medium. Bands corresponding to recombinant TIMP2 are visible in PSC secretions. No bands corresponding to recombinant TIMP1 were found in the samples. MW, molecular weight. (B) Representative western blot (n=4 separate cell preparations) for TIMP2 expression in cells after 24 hours of culture with serum free culture medium. Lane 1: Human recombinant TIMP2. Lane 2: PSC secretions.