Table 1.
Polymerase Chain Reaction Amplification Characteristics
| Microsatellite Markers | Primer Sequence | PCR Program | Size Range (bp) |
|---|---|---|---|
| AC008818-1 | F-TGCTTGGTGAAGGAATAGC
R-GTGGGTGGGTATTCATGTG |
57TD52 | 140–160 |
| D13S289 | F-CTGGTTGAGCGGCATT
R-TGCAGCCTGGATGACA |
57TD52 | 245–276 |
| D13S1238 | F-CTCTCAGCAGGCATCCA
R-GCCAACGTAATTGACACCA |
57TD52 | 129–159 |
| Single Nucleotide Polymorphisms | Primer Sequence | PCR Program | Enzyme Digest |
|---|---|---|---|
| SNP32 (rs456009) | F-TAATCTGAGCCTCAGCTTTC
R-TGTAGCTCTGGTATCATAGTGG |
57TD52 | PvuII |
| SNP45 | F-TGGCTGCAGATTACAGTG
R-AGGAGCAGGTTAAAGCAG |
57TD52 | MaeIII |
| SNP56 (rs702553) | F-ACAACAACCCTATAAGGCAG
R-ATTGTCTTGGCTATACAGGC |
57TD52 | AflIII |
| SNP83 (rs966221) | F-GATTTATGTCTTATACTTTC
R-ACGAAAAACTTCTACGTATGAAACA |
57TD52 | MaeIII |
| SNP87 (rs2910829) | F-GATGATGAGTCTGGAGCTG
R-ACTCTAACCAAGTGCTTGC |
60TD50 | SspI |
| SG13S89 | F-TGTGAAGCCCTGGAGAGGTG
R-ACCCTTGTCAGCACAGCAG |
57TD52 | AccII |
| SG13S32 | F-CCTCTCTTCTGTTCCTGG
R-ACAGGTGAACTAAACAGGATAG |
57TD52 | TaqI |
| SG13S25 | F-GACAGCATCAGCTAGTCTCTTTCC
R-GAAATTCATGTTGCTGTGTCCATACA FAM/VIC-CAGCCACTGTTRCCCA |
Assay-by-Design | |
| SG13S114 | F-CAGATGTATGTCCAAGCCTCTCT
R-AGGTAGGTCTATGGTTGCAACATTG FAM/VIC-TTGCAATTCTAWTTAACC |
Assay-by-Design |
PCR = polymerase chain reaction.