TABLE 1.
Fates of the 25 continuous evolution lineages
| Lineage designation | Bottleneck population size | Extinction at burst no. | Mutations observeda (burst appeared, burst fixed) |
|---|---|---|---|
| 4Vb | 100 | 18 | A (10, 18), F |
| 4R | 100 | 18 | A (11, 18), F |
| 4Z | 100 | 18 | A (13, 18), F |
| 4Y | 100 | 28 | A (18, 28) |
| 4W | 100 | 30 | A (22, 30) |
| 4U | 100 | 34 | A (21, 34), S (ND, ND) |
| 3K | 300 | 21 | A (15, 21), F |
| 3O | 300 | 22 | A (16, 22), F |
| 3J | 300 | 27 | A (22, 27), F |
| 3N | 300 | 31 | A (21, 31) |
| 3W | 300 | 36 | A (30, 36), S (ND, 28) |
| 3X | 300 | 37 | A (29, 37), S (ND, ND) |
| 3A | 600 | 27 | A (20, 27), F |
| 3V | 600 | 27 | A (20, 27), F |
| 3S | 600 | 31 | A (19, 31), F |
| 3E | 600 | 33 | A (25, 33) |
| 3Q | 600 | 37 | A (26, 37) |
| 5A | 600 | 50+ | M (15, 21); S (20, 43) |
| 3D | 600 | 50+ | M (ND, NF); S (ND, 32) |
| 2I | 3000 | 36 | A (18); S (7, gone by 25) |
| 2K | 3000 | 37 | A (22); S (3, 30) |
| 2A | 3000 | 45 | A (31); S (8, gone by 10) |
| 2J | 3000 | 49 | A (23, NF); M (40, NF); S (6, 30) |
| 1A | 3000 | 50+ | M (5, 10); S (6, 10) |
| 1C | 3000 | 50+ | M (5, 6); S (6, 6) |
a
A, polyadenylations; F, floodgate; M, immunity; S, insurance; NF, never fixed (always polymorphic); ND, not determined. Not all bursts were surveyed for the presence of the floodgate mutation.
b
Lineages in italics were subjected to direct sequence analyses, as well as to RFLP.