Table 2.
NHA were cultured with and without heroin (10−7 M) for 24 hr (n = 3 independent experiments using 3 different NHA cultures). Protein was extracted and subjected to DIGE as described in methods section. Data represent statistically significant differentially expressed proteins (Student’s t-test) that were identified using HPLC-MS/MS. Data are represented as protein name, gene accession number (Gi No.), theoretical isoelectric point (pI), theoretical mass and function.
| Spot # | Protein Name | Accession Number | Theoretical pI | Theoretical mass | Biological function |
|---|---|---|---|---|---|
| 1456 | protein kinase C | gi|4506067| | 4.59 | 731215.07 | kinase |
| 2434 | Myosin light chain alkali, smooth-muscle isoform | gi|16924329| | 4.56 | 16930.05 | Regulatory light chain of myosin |
| 2235 | proliferating cell nuclear antigen | gi|4505641| | 4.57 | 28768.78 | Cell cycle control protein |
| 2331 | Tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein | gi|4507953| | 4.73 | 27745.10 | signal transduction |
| 2212 | tropomyosin 3 | gi|24119203| | 4.75 | 29032.66 | Cytoskeletal protein |
| 2057 | Laminin receptor 1 | gi|9845502| | 4.79 | 32854.08 | Ribosomal subunit |
| 2398 | proteasome beta 6 subunit | gi|23110925| | 4.80 | 25357.72 | Ubiquitin proteasome system protein |
| 1942 | reticulocalbin 1 precursor | gi|4506455| | 4.86 | 38890.00 | Calcium binding protein |
| 1932 | reticulocalbin 1 | gi|4506455| | 4.86 | 38890.00 | Calcium binding protein |
| 1804 | tubulin alpha 6 | gi|14389309| | 4.96 | 49895.33 | Cytoskeletal protein |
| 2231 | chloride intracellular channel 1 | gi|14251209| | 5.09 | 26922.73 | Intracellular ligand gated channel |
| 1160 | Vimentin | gi|2119204| | 5.06 | 53651.68 | Cytoskeletal protein |
| 2417 | EF hand domain family, member D2 | gi|20149675| | 5.15 | 26697.28 | unknown |
| 2275 | Tumor protein D54 (hD54) | gi|20141658| | 5.26 | 22237.73 | regulator of cell proliferation |
| 2502 | Galectin 1 | gi|4504981| | 5.33 | 14715.70 | beta-galactoside-binding protein |
| 2432 | ATP synthase, H+transporting, mitochondrial F1 complex, delta subunit | gi|13325214| | 5.38 | 17489.96 | Transport protein |
| 2223 | cathepsin D preproprotein | gi|4503143| | 6.1 | 44552.22 | aspartic protease |
| 2374 | ribosomal protein S14 | gi|5032051| | 10.07 | 16272.71 | Ribosomal subunit |