Table 3.
NHA were cultured with and without heroin (10−7 M) for 24 hr. Protein was extracted and subjected to DIGE as described in Materials and Methods. Data represent statistically significant up and down regulated spots that were identified using HPLC-MS/MS (n = 3 independent experiments using 3 different NHA cultures, Student’s t-test).
| Spot Number | Protein Name | Volume Ratio | P value |
|---|---|---|---|
| Up Regulated Proteins | |||
| 1456 | protein kinase C | 2.14 | 0.001 |
| 1942 | reticulocalbin 1 precursor | 1.96 | 0.05 |
| 1932 | reticulocalbin 1 | 1.52 | 0.05 |
| 2231 | chloride intracellular channel 1 | 2.58 | 0.001 |
| 2223 | cathepsin D preproprotein | 1.89 | 0.001 |
| 2331 | tyrosine 3-monooxgenase/tryptophan 5-monooxgenasea ctivation protein | 3.85 | 0.0097 |
| 2434 | Myosin light chain alkali, smooth-muscle isoform | 2.62 | 0.024 |
| 2502 | galectin 1 | 1.49 | 0.022 |
| Down Regulated proteins | |||
| 2235 | proliferating cell nuclear antigen | −1.82 | 0.033 |
| 2212 | tropomyosin 3 | −1.49 | 0.0063 |
| 2398 | proteasome beta 6 subunit | −1.45 | 0.01 |
| 2057 | laminin receptor 1 | −1.68 | 0.0029 |
| 1804 | tubulin alpha 6 | −1.58 | 0.0035 |
| 1160 | vimentin | −1.74 | 0.05 |
| 2417 | EF hand domain family member D2 | −2.36 | 0.0056 |
| 2275 | Tumor protein D54 (hD54) | −1.44 | 0.035 |
| 2432 | ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit | −2.83 | 0.0069 |
| 2374 | ribosomal protein S14 | −1.54 | 0.0051 |