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The activities of uncleaved UmuD and UmuD′ in combination with UmuC increase resistance to DNA damage. (A) Survival after UV irradiation of isogenic strains of GW8024 [recA+ Δ(umuDC)595∷cat lexA(Def)] (20) that differ in the genotype of umuD and umuC carried on a plasmid: (■) umuD+ umuC+ [pSE117] (25); (▵) umuD′ umuC+ [pGW3751] (56); (▴) umuD(S60A) umuC+ [pTO4] (this work); (□) ΔumuDC [pBR322kan] (20). (B) Same as in A, but strains are GW8023 [recA+ Δ(umuDC)595∷cat lexA+] (20).
