Table 1.
Summary of H19–lacZ, H19–PLAP, and DEL–H19 transgenic lines
| Upstream region, kb | Transgene | Line | Copy no. | Transgenic embryos, total no. | Imprinting | Differential methylation |
|---|---|---|---|---|---|---|
| −10.5 | H19–PLAP | FL3 | 1 | 26 | No | No |
| FL5 | 7 | 56 | Yes | Yes | ||
| FL8 | 9 | 32 | Yes* | ND | ||
| FL15 | 1 | 43 | No | Yes | ||
| FL16 | 14 | 47 | Yes | Yes | ||
| HGF20 | MLK16.1 | 4 | 22 | Yes | Yes | |
| HGF23 | 3 | 2 | 34 | Yes | ND | |
| 16b | 1 | 41 | Yes | ND | ||
| −3.7 | HGF22 | 3.7RF3y | 6 | 14 | Yes | Yes |
| −0.2 | HGF21 | SCB01 | 5 | 3 | No | NA |
| SCB02 | >50 | 10 | No | NA | ||
| SCB10 | 10 | 5 | No | NA | ||
| −9.3 | DEL–H19 | DEL7 | 1 | 35 | No* | Yes |
| DEL16 | 10 | 23 | No* | Yes | ||
| DEL21 | 5 | 11 | No | Yes | ||
| DEL24 | 4 | 21 | No | Yes |
The copy number, total number of transgenic embryos examined, imprinting as judged by repression on paternal transmission, and differential methylation of the DMD region are indicated for each line. Nine H19–lacZ lines were excluded from the analysis because of failure of transmission or expression. Transgenes from line HGF20-23 each contain the lacZ reporter, but differ in the amount of upstream region. Asterisks in “Imprinting” column mark lines that showed some anomalous repression as described in the text. ND, not done. NA, not applicable.