Figure 3.

Growth effects of wild-type and naturally occurring mutant alleles of LKB1 in G361 melanoma cells. (A) Western blotting analysis of Lkb1 in cells transfected with indicated expression vectors. Detection was performed with anti-Lkb1, except for Lkb1-SL8, which was detected by antihemagglutinin because of a C-terminal truncation. (B) Photographs of Giemsa-stained G418-resistant colonies of G361 cells from plates transfected with indicated expression vectors. (C) Relative numbers of G418-resistant colonies after transfections with the indicated expression vectors shown in B. Standard deviations are from nine (vector, Lkb1) or six (G163D, SL26, SL8) independent experiments.