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DNA methylation analyses. (a) Digestion of DNA with EcoRI and MluI and hybridization with a Snurf–Snrpn exon 1 probe identifies bands corresponding to the methylated maternal (M) and unmethylated paternal (P) alleles in wild-type mice. Paternal or maternal inheritance of the transgene yields only the maternal or paternal methylation pattern, respectively. (b) Similar results are seen for Zfp127 (XbaI–EagI), mapping 1 megabase distal of Snrpn.
