Figure 1.

Generation of NKDT chimeric mice. (A) A partial map of the NK1.1 gene locus and the targeting construct. The targeting vector in pBluescript II SK (Stratagene) was constructed by replacing the 3′ downstream from the NK1.1 initiation codon with the DT-A (DT) and neo^r (Neo^r) genes. Restriction-enzyme-sensitive sites are indicated as follows: B, BamHI; E, EcoRI; K, KpnI; X, XhoI. (B) Southern blot analysis of the represented ES clone with specific homologous recombination. The genomic DNA was digested with BamHI and hybridized with probe A (5′) and probe B (3′) as indicated in A. Molecular sizes (kb) of the bands are indicated at the margin. +/+: wild-type, +/−: heterozygotes.