. Author manuscript; available in PMC: 2007 Jan 18.

Published in final edited form as: J Biomater Sci Polym Ed. 2006;17(10):1141–1158. doi: 10.1163/156856206778530731

Table 1.

Rho GTPase activation summary for (monocyte-) macrophage cell lines

		Cell line
Protein	Culture surface	RAW 264.7	J774A.1	IC-21
Rho	TCPS	+	+	−
	PS	+	+	−
	PLA	+	+	−
	Teflon^® AF	+	+	−
Rac1	TCPS	−	+	+
	PS	−	+	−
	PLA	−	+	−
Cdc42	Teflon^® AF	−	+	−
	TCPS	+	−	+
	PS	+	−	−
	PLA	+	−	+
	Teflon^® AF	+	−	−

⁺

indicates the presence of the GTP-bound form of the protein(s), −indicates the absence of the GTP-bound form of the protein(s). The Rho pulldown and probe is not specific for RhoA, but rather detects Rho isoforms A, B and C. See Materials and Methods for a detailed explanation.