Figure 4.

Similar intragenic suppression phenotype is seen for analogous mutations in E. coli dnaK. pQE60 vector or pBB46 containing wt or mutant dnaK under the control of an isopropyl β-d-thiogalactoside (IPTG)-inducible promoter was transformed into strain BB205. Strains were grown overnight at 30°C, a permissive growth temperature for dnaK mutants. Serial dilutions (1:10) were made, equivalent numbers of cells were plated on media containing 15 μM IPTG, and cells were grown overnight at the indicated temperatures.