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DnaJ stimulation of DnaK ATPase activity. Single-turnover ATPase assays were performed in the absence (□) or presence (♦) of DnaJ. DnaK concentration is ≈0.35 μM; DnaJ is 0.2 μM. Fold stimulation of ATPase activity by DnaJ is shown in the box. (A) wt DnaK. (B) I462T. (C) D79A-I462T. (D) D79A.
