Figure 1.

The virus-specific CD8⁺ T cell response in mice primed with recombinant vaccinia viruses expressing the p56 and p79 epitopes of γHV-68. The B6 mice were infected i.p. with vacc-p56, vacc-p79, or the control vacc-LCMV 1 month before i.n. challenge with γHV-68. Pooled BAL or PBL populations from three to six mice were then analyzed for the prevalence of p56D^b+CD8⁺ (A and C) or p79K^b+CD8⁺ (B and D) T cells. Virus titers (mean of three to six samples) were determined by limiting-dilution assay for lungs (closed symbols) taken on day 7 and for spleens (open symbols) sampled through the course of the experiment (E). Both priming regimes significantly decreased (P < 0.005) the level of infection in the spleen at the day-17 time point.