Table 5.

Cross-immunization experiments

Immunizing peptide	% of peptide-positive hybridomas	% of peptide-specific hybridomas responding to GAD65 protein
456–470 (PD epitope)	448/405  =  89%	0/41  =  0%
331–345 (PD epitope)	458/504  =  91%	0/48  =  0%
206–220 (g7 epitope)	144/168  =  85%	40/48  =  83%

NOD mice were immunized with two PD epitopes (p456–470 or p331–345), 50 μg emulsified in complete Freund’s adjuvant (CFA), to generate PD epitope-specific. I-A^g7 restricted T cell hybridomas. In another group, NOD mice were immunized with a g7 epitope (p206–p220), 50 μg in CFA, to generate p206–220-specific, I-A^g7-restricted T cell hybridomas. The percentage of peptide-positive hybridomas represents the ratio of the number of peptide-responding T cell hybridomas to the total number of screened hybridomas. The percentage of peptide-specific hybridomas responding to GAD65 protein represents the ratio of the number of peptide-responding T cell hybridomas responding to GAD65 to the total number of peptide-responding T cell hybridomas that were screened.