CENP-E regulation. The figure shows CENP-E messenger RNA in CENP-E high (right lanes 3/4, 7/8, 11/12 and 15/16) and CENP-E low (left lanes 1/2, 5/6, 9/10 and 13/14) expressing rheumatoid arthritis synovial fibroblast populations after different prednisolone exposure (lanes 1–8) and serum starvation (lanes 9–12). As compared with unstimulated fibroblasts (lanes 13–16) no significant alteration in CENP-E messenger RNA expression, either in CENP-E high- or in CENP-E low-expressing fibroblast populations, can be observed after prednisolone exposure. Serum starvation was also not able to suppress CENP-E messenger RNA completely (lanes 9–12).