Figure 5.

CpG induces MHC II, CD86, and CD83 on CD4-negative peripheral blood dendritic precursor cells. Lineage-positive cells and CD4-positive dendritic precursor cells were depleted from PBMC by immunomagnetic cell sorting. Remaining cells were incubated with GMCSF (800 units/ml) and the CpG oligonucleotide 2006 (2 μg/ml) as indicated. After 72 h, cells were stained with MHC II (differentiation marker), CD86 (costimulatory molecule), and CD83 (maturation marker) and examined by flow cytometry. (A) MHC II and CD86 expression is depicted. (B) Expression of CD83 on MHC II-positive cells is analyzed.