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. 1999 Aug 3;96(16):9322–9327. doi: 10.1073/pnas.96.16.9322

Figure 1.

Figure 1

In vivo ovarian lipid accumulation in and in vitro development of preimplantation embryos from wild-type and SR-BI KO mice. Six-week-old female mice were superovulated and were mated to males of the other genotype (i.e., SR-BI+/+ females mated to SR-BI−/− males and vice versa) to generate embryos with heterozygous mutant genotypes. Ovaries and preimplantation embryos were harvested the next morning (day 0). (A and B) Typical oil red O staining of lipids in ovaries from SR-BI+/+ (A) or SR-BI−/− (B) animals. The arrows indicate corpora lutea. (Bar = 450 μm.) (C and D) Phase-contrast microscopy of preimplantation embryos (cultured for 1 day) from SR-BI+/+ (C) or SR-BI−/− (D) females mated to males of the opposite genotype. Similar results were observed when SR-BI−/− males were mated to SR-BI−/− females. Open arrowheads indicate morphologically normal, one- or two-cell embryos; solid arrowheads indicate embryos with abnormal, nonrefractive morphology. (Bar = 100 μm.) (E) Plasma progesterone concentrations from pseudopregnant females (6 days postmating, ages 6–10 weeks, weight = 19–25 g, n = 8; P = 0.08). (F) Percentage of preimplantation embryos from SR-BI+/+ (open bars) or SR-BI−/− (solid bars) females with normal morphology during 3 days of culture. The values represent the averages from five animals of each genotype. Total number of embryos: SR-BI+/+, 131; SR-BI−/−, 167.