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. 1999 Aug 3;96(16):9339–9344. doi: 10.1073/pnas.96.16.9339

Figure 5.

Figure 5

Passaging of HK666 leads to induction of BZLF-1 and loss of Qp expression. (A) Expression of Qp-initiated EBNA-1 transcripts and BZLF-1 transcripts in HK666 cells was examined by RT-PCR. Southern blots of the DNA products were probed for BKRF-1 (EBNA-1) (Upper) and BZLF-1 (Lower). Cells at passage 4 were also tested after treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA) (20 ng/ml) and n-butyrate (3 mM) for 48 hr (+TPA). (B) Detection of Zta protein in HK666 cells by immunoperoxidase staining.