Figure 1.

Integration of pSR1 into the S. aureus chromosome. Black: fmhB gene; hatched: xylose regulon (xylR and the operator region of xylA); white: fmhB promoter region; bold line: pOX7 plasmid DNA. Relevant restriction sites are indicated as follows: H, HindIII; B, BamHI; K, KpnI; E, EcoRI. Primers used for cloning and PCR controls are indicated by arrows and numbered as in Materials and Methods. The region spanned by the internal DNA probe used for Southern and Northern blots is indicated by a line above fmhB. Not to scale.