Figure 3.

Muropeptide profiles. Isolated peptidoglycan was digested with muramidase and muropeptides were subjected to reversed-phase HPLC. Strain BB270 grown at 42°C (a) with xylose, (b) with glucose. Strain SR18 grown (c) with xylose, (d) with glucose. The muropeptide fractions are indicated as follows: monomers, corresponding to uncrosslinked subunits (retention time 10–45 min); dimers, corresponding to two crosslinked muropeptides (45–85 min); trimers, corresponding to three crosslinked muropeptides (85–105 min); and oligomers, corresponding to three or more crosslinked muropeptides. More highly crosslinked muropeptides in the oligomeric fraction are indicated by numbers.