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hrp-dependent secretion of AvrRxv. Total protein (lanes 1–4) and supernatants (lanes 5–8) of 82*(pLAFR3) (lanes 1 and 5), 82*ΔhrcV(pLAFR3) (lanes 2 and 6), 82*(pLRF) (lanes 3 and 7), and 82*ΔhrcV(pLRF) (lanes 4 and 8) were separated by SDS/PAGE (10% polyacrylamide) and analyzed by immunoblotting by using anti-Flag antibody (Upper) or anti-HrcN antibody (Lower). The molecular mass of the proteins is indicated by an arrow.
