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Luciferase reporter assay of the NOS2 promoter with gene polymorphisms. The (a) pGL3-T vectors and (b) pGL3-G vectors were cloned by the NOS2 promoter region, including alleles T and G of the single nucleotide polymorphism at -1026 bp. In the series of pGL3-T or pGL3-G vectors, the 6, 8, 10, 12 and 14 repeats of the CCTTT region were cloned upstream of the inserted NOS2 promoter in pGL3-T or pGL3-G. bp, base pairs; IL, interleukin.
