Table 1.
IIF | K562 cell extract IP | TnT IP | |||
Sera | GWB foci | 100/102-kDa Su | 200-kDa Su | hAgo2 | Dicer |
Human anti-Su (n = 22) | 91% | 100% | 82% | 91% | 36% |
Mouse anti-Su (n = 7) | 100% | 100% | 100% | 100%a | 0%b |
Human anti-GW182 (n = 2) | 100% | 50% | 50% | 50% | 100% |
Human autoimmune non-anti-Su (n = 10) | 0% | 0% | 0% | 0% | 0%c |
Normal human (n = 10) | 0% | 0% | 0% | 0% | 0%c |
Pre-immune mouse (n = 6) | 0% | 0% | 0% | 0%c | 0%d |
an = 5, bn = 2, cn = 4, dn = 1. GWB, GW body; IIF, indirect immunofluorescence of HEp-2 cells; IP, immunoprecipitation; TnT, in vitro transcription and translation.