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. Author manuscript; available in PMC: 2007 Jan 19.
Published in final edited form as: Mol Cell Endocrinol. 2006 Nov 13;263(1-2):181–189. doi: 10.1016/j.mce.2006.09.012

Figure 3.

Figure 3

PGE2 increases cyp19 promoter activity; Effect of 5’ deletions. A, Undifferentiated granulosa cells were transfected with cyp19 promoter reporter-constructs containing 5′-serial deletions. Twenty-four hours later, cells were treated with PGE2 (1 μM) or vehicle for 8 hours. B, Cells were transfected with the -245cyp19pr-luc promoter construct and 24 hours later treated with either vehicle or increasing concentrations of PGE2 for 8 hours. Transient expression of the reporter gene was quantified by a standard luciferase bioluminescence assay and normalized against β-galactosidase. Bars represent mean ± SEM of 3 independent experiments each one performed in triplicate. *** p<0.001 vs. cells treated with vehicle (ANOVA I - Tukey test).