Table 1.
Proteins identified | ||||||
Proteomic approach | Protein amount | Number of fractions | Unique peptides | 1 Upep | >2 Upeps | Total |
LC-MS (MudPIT) | 1.4 mg | 45 | 5,540 | 636 | 848 | 1,484 |
LC/LC-MS/MS | 1.0 mg | 80 | 7,537 | 513 | 991 | 1,504 |
GeLC-MS/MS | 0.1 mg | 20 | 20,893 | NA | 2,003 | 2,003 |
MudPIT refers to Washburn et al. [14], LC/LC-MS/MS refers to Peng et al. [15] and GeLC-MS/MS refers to work presented in this study. NA, not applicable; Upep, unique peptide.