Table 3.
Genes selected for validation by qPCR from microarray analyses (supplemental table).
| Gene Name | Accession # | Bases Spanned | Annealing Temperature | Experiments* |
| 7-dehydrocholesterol reductase | NM_001360 | 127-277 | 58°C | 5 |
| Activator of heat shock 90kDa protein ATPase homolog 1 | NM_012111 | 494-621 | 60°C | 5 |
| Adrenomedullin | NM_001124 | 40-148 | 55°C | 5 |
| Aldolase C, fructose-biphosphate | NM_005165 | 60-252 | 60°C | 5 |
| Alpha tubulin-like | NM_145042 | 686-848 | 62°C | 5 |
| bHLH-PAS type transcription factor NXF | NM_153553.1 | 388-578 | 58°C | 1 & 2 |
| Calpain 10 | NM_011796 | 844-1626 | 64°C | 1 & 2 |
| Calponin 1 | NM_009922 | 303-771 | 62°C | 1 & 2 |
| CCAAT/enhancer binding protein, beta | NM_009883 | 1174-1305 | 58°C | 1 & 2 |
| CCAAT/enhancer binding protein, delta | NM_007679 | 1278-1434 | 60°C | 3 |
| CD52 antigen | NM_013706 | 52-234 | 62°C | 3 |
| c-FOS | NM_010234 | 36-390 | 62°C | 1, 2, 3, & 4 |
| Chemokine (C-X-C motif) receptor 4 | NM_009911 | 158-288 | 58°C | 4 |
| Cofilin 1 | BB097704 | 55-273 | 58°C | 3 |
| Cold inducible RNA binding protein | NM_007705 | 221-349 | 58°C | 1 & 2 |
| Cyclin G2 | NM_004354 | 124-271 | 60°C | 5 |
| Cytochrome P450, family 1, subfamily a, polypeptide 2 | NM_009993 | 489-609 | 62°C | 4 |
| Cytochrome P450, family 2, subfamily a, polypeptide 4 | NM_009997 | 458-628 | 55°C | 4 |
| Cytochrome P450, family 2, subfamily f, polypeptide 2 | NM_007817 | 236-408 | 55°C | 4 |
| Cytochrome P450, family 51, subfamily a, polypeptide 1 | NM_000786 | 10-172 | 60°C | 5 |
| DNA damage inducible transcript 4 | NM_019058 | 365-499 | 60°C | 5 |
| Dystrobrevin beta | NM_007886 | 1496-1838 | 62°C | 1 & 2 |
| Fatty acid desaturase 1 | NM_013402 | 359-514 | 60°C | 5 |
| FK506 binding protein 11 | NM_024169 | 253-644 | 62°C | 1 & 2 |
| G protein-coupled receptor 114 | NM_153837 | 150-284 | 58°C | 5 |
| G1p2, Isg15 ubiquitin-like modifier | NM_015783 | 107-234 | 58°C | 3 |
| Glucocorticoid induced gene 1 | NM_133218 | 1236-1566 | 55°C | 1 & 2 |
| Glutamate receptor, ionotropic, kainate 5 | NM_008168 | 911-1769 | 62°C | 1 & 2 |
| Glutamine Synthetase | X16314.1 | 309-829 | 62°C | 1 & 2 |
| Granzyme A | NM_010370 | 187-475 | 62°C | 3 |
| Growth arrest and DNA-damage-inducible 45 beta | NM_008655 | 351-506 | 60°C | 1, 2, & 3 |
| Growth arrest and DNA-damage-inducible 45 gamma | NM_011817 | 225-348 | 55°C | 1 & 2 |
| Growth Hormone | NM_008117.2 | 347-447 | 58°C | 1 |
| Intercellular adhesion molecule 2 | NM_000873 | 85-207 | 60°C | 5 |
| Interferon regulatory factor 7 | AK079685 | 703-837 | 60°C | 3 |
| Interleukin 21 receptor | NM_021798 | 213-406 | 58°C | 5 |
| Internexin neuronal intermediate filament protein | NM_010563 | 1022-1281 | 55°C | 1 & 2 |
| Jun dimerization protein 2 | NM_130469 | 44-211 | 60°C | 5 |
| Jun-B | NM_008416 | 994-1214 | 62°C | 1 & 2 |
| KRAB zinc finger protein KR18 | XM_139814 | 108-206 | 60°C | 4 |
| Mitogen-activated protein kinase kinase kinase 6 | NM_016693 | 677-791 | 62°C | 1, 2, & 4 |
| Neural Proliferation, differentiation and control gene 1 | NM_008721 | 200-586 | 62°C | 1 & 2 |
| Neurotransmitter transporter, creatine Slc6a8 | NM_133987 | 2203-2499 | 60°C | 1 & 2 |
| Neurotransmitter transporter, glycine Slc6a9 | NM_008135 | 67-285 | 58°C | 3 |
| Nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha | NM_010907 | 292-467 | 58°C | 1 & 2 |
| Nucleoporin 88kDa | NM_002532 | 65-218 | 60°C | 5 |
| pEL98 protein | D00208 | 231-341 | 60°C | 3 |
| Peroxiredoxin 3 | NM_007452 | 86-488 | 62°C | 1 & 2 |
| Pigpen protein | AK086587 | 529-722 | 60°C | 1 & 2 |
| Placenta-specific 8 | NM_139198 | 88-196 | 58°C | 3 |
| Programmed cell death 10 | NM_019745 | 508-791 | 58°C | 1 & 2 |
| Prostaglandin-endoperoxide synthase 2 | NM_011198 | 1231-1410 | 62°C | 1 & 2 |
| RAB1 | NM_008996 | 81-237 | 58°C | 1 & 2 |
| RAB33A | NM_004794 | 344-510 | 58°C | 5 |
| S100 calcium binding protein A8 | NM_013650 | 245-348 | 55°C | 3 |
| S100 calcium binding protein A9 | NM_009114 | 35-174 | 62°C | 3 |
| Serum/glucocorticoid regulated kinase | NM_011361 | 158-329 | 60°C | 1, 2, & 4 |
| Spindle pole body component 24 homolog | NM_182513 | 381-486 | 60°C | 5 |
| Squalene epoxidase | NM_003129 | 609-785 | 60°C | 5 |
| SRY-box containing gene 2 | NM_011443 | 633-756 | 55°C | 4 |
| Thioredoxin 1 | NM_011660 | 88-335 | 55°C | 1 & 2 |
| Transcription factor 7-like 2 | NM_030756 | 345-496 | 60°C | 5 |
| Transformation related protein 53 inducible nuclear protein 1 | NM_021897 | 236-416 | 58°C | 4 |
| Tubulin, alpha 4 | NM_009447 | 94-310 | 60°C | 1, 2, 3, & 4 |
| Tumor necrosis factor (ligand) superfamily, member 13b | NM_033622 | 661-1056 | 65°C | 1 & 2 |
| Tumor protein p53 inducible nuclear protein 1 | NM_033285 | 1883-2040 | 58°C | 5 |
| UDP-glucuronic acid decarboxylase | AK005536 | 570-706 | 58°C | 4 |
| X-box binding protein 1 | NM_005080 | 610-715 | 60°C | 5 |
| Following microarray analyses, genes were chosen based on fold change and p-value for validation by qPCR. GenBank accession numbers are listed. *1: Mouse brain DA TC, 2: Mouse brain DA DR, 3: Mouse blood DA TC, 4: Mouse brain PbTx TC, 5: Human AZA TC. DA: domoic acid. PbTx: brevetoxin. AZA: azaspiracid. TC: time course. DR: dose response. | ||||