Table 1.
Distribution of 14C isotope in sugar phosphates isolated from spinach leaves following specified intervals of PS in 14COa2
| Carbon | 10 s of 14CO2 fixation | 45 s | 75 s | 120 s | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Glc 6-P | d-g-d-i-oct 1,8-P2 | Rib 5-P | PGA | Glc 6-P | d-g-d-i-oct 1,8-P2 | Glc 6-P | d-g-d-i-oct 1,8-P2 | Glc 6-P | |||||||||||
| Found | Predicted | ||||||||||||||||||
| 1 | 3.2 | 35.6 | (33.3) | 23.4 | 84.9 | 12.7 | 20.4 | (38.7) | 15.1 | 29.9 | (37.3) | 18.5 | |||||||
| 2 | 3.2 | 25.3 | (33.3) | } | 7.2 | 7.4 | 21.1 | (22.6) | 10.3 | 30.6 | (25.4) | 11.8 | |||||||
| 3 | 37.2 | 39.1 | (33.3) | 71.6 | 7.9 | 24.6 | 58.5 | (38.7) | 22.0 | 39.5 | (37.3) | 17.2 | |||||||
| 4 | 50.5 | 21.2 | (3.1) | 38.1 | 13.1 | (8.5) | 33.4 | 13.3 | (12.1) | 25.0 | |||||||||
| 5 | 2.5 | } | } | 5.0 | 7.0 | } | } | 9.1 | } | } | 10.7 | ||||||||
| 6 | 3.4 | (60.7) | (93.2) | 10.2 | 79.2 | (79.8) | 10.1 | 63.7 | (76.0) | 17.1 | |||||||||
| 7 | |||||||||||||||||||
| 8 | 18.1 | (3.5) | 7.7 | (11.7) | 23.0 | (11.9) | |||||||||||||
| % recovery of 14C-isotope | 88 | 91b | 76c | 86 | 101 | 106 | 82b | 75c | 93 | 85b | 89c | 90 | |||||||
aThe conduct of the experiments is described in the Methods section. Sugar phosphates were isolated and purified by ion-exchange and paper chromatographic procedures. Results are mean values of duplicate degradations except for all of the compounds isolated after 10 s of 14CO2 fixation, which were degraded in triplicate. b, c% 14C recovered in the top 3 and bottom 5 carbons of d-g-d-i-oct 1,8-P2