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RT-PCR method used to evaluate mRNA expression levels of adult MHC isoforms and α-actin mRNA in the untreated right legs of IRR (IRR-control), sham-irradiated (IRR), irradiated and stimulated (IRR-Stim), and stimulated (Stim) rat tibialis anterior muscles. Sequences were amplified using a multiplex assay format for individual MHC isoforms with the internal control α-actin.
