Figure 3. Effect of acute hyperinsulinaemia on signalling protein phosphorylation in fetal skeletal muscle in utero.

The results show a robust insulin response to stimulate IR, IRS-1, p85-PI 3-Kinase, Akt and p70S6 kinase in fetal skeletal muscle in utero during a hyperinsulinaemic–euglycaemic clamp. Repeated muscle biopsies were taken in control (saline-infused) fetuses and in twin fetuses clamped over 240-min period (n = 3 animals/group). From Anderson et al. (2005), with permission.