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. 2006 Mar 9;573(Pt 1):35–43. doi: 10.1113/jphysiol.2006.108092

Figure 5. Ca2+ permeability of human mutant AChRs.

Figure 5

A, simultaneous recordings of whole-cell current (top) and Ca2+ transient (bottom), induced by nicotine (100 μm, horizontal thin line), in GH4C1 cells transiently transfected with αV249Fβɛδ (left) and αG153Sβɛδ (right) AChR subunit cDNAs. Holding potential, −70 mV. Traces are aligned and share the same temporal scale. B, simultaneous recordings of whole-cell current (top) and Ca2+ transient (bottom), induced by nicotine, in GH4C1 cells transiently transfected with αV249Fβγδ (left) and αG153Sβγδ (right) AChR subunit cDNAs. Holding potential, −70 mV. Traces are aligned and share the same temporal scale. C, linear relationships between ΔF/F and Q obtained from the cells shown in A and B. For these examples, the Pf values, calculated by normalizing the slopes obtained in A and B (standard medium) with respect to the mean slope obtained from calibration experiments (8.0 ± 0.4 nC−1, dashed line), are 7.7% (αV249Fβɛδ, ▵), 7.6% (αG153Sβɛδ, □), 3.7% (αV249Fβγδ, ▴), and 3.4% (αG153Sβγδ, ▪).