Table 5.

Relative frequencies of mono- and dinucleotides in 5-AzaC modulated genes and in the whole genome.

		Whole genome		Up-regulated genes		Down-regulated genes
Nucleotide		Coding	Promoter	Coding	Promoter	Coding	Promoter
Mononucleotide	A	0.36	0.40	0.42	0.40	0.40	0.40
	C	0.14	0.11	0.11	0.11	0.11	0.11
	G	0.14	0.12	0.16	0.12	0.15	0.12
	T	0.36	0.36	0.31	0.37	0.34	0.36
Dinucleotide	AA	0.14	0.19	0.18	0.18	0.17	0.19
	AC	0.04	0.04	0.04	0.04	0.04	0.04
	AG	0.05	0.05	0.06	0.05	0.06	0.05
	AT	0.12	0.12	0.12	0.13	0.13	0.12
	CA	0.06	0.05	0.06	0.05	0.06	0.05
	CC	0.02	0.01	0.02	0.01	0.01	0.01
	CG	0.01	0.01	0.01	0.01	0.01	0.01
	CT	0.05	0.04	0.03	0.04	0.04	0.04
	GA	0.06	0.06	0.08	0.05	0.07	0.05
	GC	0.01	0.01	0.01	0.01	0.01	0.01
	GG	0.02	0.02	0.02	0.01	0.02	0.01
	GT	0.04	0.04	0.04	0.04	0.04	0.04
	TA	0.09	0.11	0.09	0.11	0.10	0.11
	TC	0.06	0.05	0.04	0.05	0.04	0.05
	TG	0.06	0.05	0.06	0.05	0.06	0.05
	TT	0.14	0.15	0.11	0.16	0.13	0.16

The frequency of each mono and di-nucleotide was calculated for the entire genome and for the coding and promoter regions of the genes up and down-regulated by 5-AzaC. No specific association of any given mono or di-nucleotide frequency with genes that are responsive to 5-AzaC compared to the whole genome was identified.