FIGURE 6.
Plot of measured interaction fractions as a function of incubation time for a secondary FITC anti-mouse IgG (Fab-specific), and a secondary Alexa 633 anti-mouse IgG (Fc-specific) incubated on a coverslip coated with mouse monoclonal IgG. Each point is an average of 10 dual color image analyses recorded from different regions of the sample. Error bars are propagated standard errors of the mean.