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. 2006 Sep 29;91(12):4519–4527. doi: 10.1529/biophysj.106.093641

FIGURE 2.

FIGURE 2

Comparison of spectroscopic and photochemical properties of full-length and truncated ASR. (A) Difference absorption spectra of dark-adapted minus light-adapted purified pigments in detergent. (B) Kinetics of dark adaptation in purified pigments. Absorption changes were normalized to the absorption of dark-adapted samples. (C) Laser flash induced absorption changes of purified pigments at different wavelengths, monitoring the unphotolyzed state (550 nm) and M intermediate (400 nm). (D) Formation and decay of M intermediate pigments in intact E. coli cells.