TABLE 1.
Overview of the biophysical parameters for the different mutations analyzed
| Construct | Sequence | Net charge | E1/2 (mV) | k (mV) | ΔG0 (Kcal/mol) | n |
|---|---|---|---|---|---|---|
| WT | K I K E R | +2 | 1.8 ± 1.8 | 7.3 ± 0.2 | 0.13 ± 0.1 | 14 |
| Δ171-372 | N.A. | 0.3 ± 1.5 | 7.8 ± 0.3 | 0.02 ± 0.1 | 20 | |
| Δ143-372 | N.A. | −48.1 ± 1.4* | 7.8 ± 0.6 | −3.37 ± 0.3 | 18 | |
| Δ143-170 | N.A. | 11.3 ± 1.6* | 7.2 ± 0.2 | 0.86 ± 0.1 | 6 | |
| Δ143-361 | N.A. | −3.1 ± 2.0 | 10.2 ± 0.5* | −0.17 ± 0.1 | 11 | |
| EIEEE | E I E E E | −4 | −42.9 ± 0.9* | 5.9 ± 0.4* | −3.96 ± 0.3 | 17 |
| AIAAA | A I A A A | −10.8 ± 2.1* | 8.1 ± 0.2* | −0.73 ± 0.1 | 5 | |
| K362E | E I K E R | 0 | −10.8 ± 1.9* | 8.3 ± 0.4* | −0.71 ± 0.1 | 9 |
| K364E | K I E E R | 0 | −12.1 ± 1.5* | 7.6 ± 0.2 | −0.87 ± 0.1 | 11 |
| R366E | K I K E E | 0 | −20.0 ± 2.1* | 8.1 ± 0.3* | −1.35 ± 0.2 | 12 |
| E365R | K I K R R | +4 | 18.8 ± 0.6* | 7.7 ± 0.2 | 1.33 ± 0.1 | 7 |
The local net charge, activation midpoint (E1/2), slope factor (k), Gibbs free energy (ΔG0) and n the number of cells analyzed are represented for each mutation.
*
p < 0.05 compared to WT; N.A. not applicable.