Skip to main content
. Author manuscript; available in PMC: 2007 Mar 1.
Published in final edited form as: Exp Parasitol. 2006 Oct 17;115(3):310–313. doi: 10.1016/j.exppara.2006.09.008

Fig. 1.

Fig. 1

Generation of P. yoelii parasite mutant lines that express GFP. (A) Correct integration of the vector in PyGFP as shown by PCR. Lane 1: Detection of DNA of wild type (Pywt) parasites (primers PyL739 (5'-ATGTAATATTTGGATATTTC) and PyL740 (5'-TCACCTACGGAAACCTTGTTAC)); lane 2: verification of PyGFP 5' integration site (primers PyL739/L635(de Koning-Ward et al., 1998)); lane 3: verification of PyGFP 3' integration site (primers L665(de Koning-Ward et al., 1998)/PyL740); lane 4: amplification of gfpmut3 (primers GFPFNh (5'-GCTAGCAGTAAAGGAGAAGAACTTTTCA)/GFPRNh (5'-TCGCTAGCTTTGTATAGTTCATCCATGC); lane 5: negative control. (B) Schematic representation of integrated pL0016 vector into the c-rrna unit (Franke-Fayard et al., 2004).