TABLE 1.

Quantifications on 3D guard cell objects

	Volume (μm3)	Surface area (μm2)	Aperture (μm)	Pore area (μm2)
Closed	3691.06 ± 478.40	1651.59 ± 126.48	5.97 ± 0.64	123.88 ± 20.35
Open	4603.76 ± 615.76	1899.29 ± 154.25	12.54 ± 0.56	262.14 ± 12.13
Δ (open-closed)	912.70 ± 238.17	247.70 ± 47.08	6.58 ± 0.65	138.27 ± 23.71
Factor (open/closed)	1.25 ± 0.06	1.15 ± 0.03	2.12 ± 0.22	2.17 ± 0.41

The values were obtained from quantifications on 3D reconstructions (volume and surface area, Fig. 2, e and g) and equatorial sections (aperture and pore area, Fig. 2, a and b) of 10 intact guard cells in their closed and open states.