Phenotypes of wild-type (wt) plants, plants with one of two rot3 alleles, and transgenic rot3-1 plants that overexpressed ROT3. (A) The leaves in each row were taken from the following plants: (from left) wt, rot3-1, rot3-2, transgenic plants that overexpressed the wild-type ROT3 gene (A-1, A-3, and A-4), and a plant that expressed the ROT3G80E gene (G80E). Upper and lower rows show the first leaves and the fifth leaves, respectively. (Bar = 10 mm.) Paradermal images of palisade cells in the fifth leaves of a wt plant (B), a rot3-1 mutant plant (C), and an A-3 transgenic plant (D) at the fully expanded stage. (Bar = 100 μm.) Flowers of a wt plant (E), a rot3-1 mutant plant (F), and an A-1 transgenic plant (G). (Bar = 1 mm.) Gross morphology of plants heterozygous for the transgene (InducibleP∷ROT3G80E). Heterozygous 17-day-old plants (H) were transferred to medium plus 10 μM dexamethasone (I) or to control medium (J) and photographed 8 days later (I and J). (Bars = 10 mm.)