Figure 3.

Amplification by RT-PCR of ROT3 mRNA. (From left) Nontransgenic wild type (wt), nontransgenic rot3-1 null mutant (rot3-1), and three independent transgenic lines that carry the InducibleP∷ROT3 chimeric gene are shown. Plants were cultivated with (+) or without (−) treatment with 10 μM dexamethasone (DEX). Total RNA (1 μg) was amplified for each strain. (Upper) The result of amplification by RT-PCR of the ROT3 cDNA. (Lower) The result for amplification by RT-PCR of β-tubulin 4 (TUB4) fragments is shown for standard controls.